Figure 1. ASPP1/2 interact with multiple kinetochore components.

a. Tandem affinity purification of ASPP1/2-containing protein complexes were conducted using MOCK HeLa cells or cells stably expressing FLAG-HA (FH)-ASPP1 or ASPP2. Associated proteins were separated by SDS-PAGE and visualized by Coomassie Blue(CB)staining. The proteins and the number of peptides identified by mass spectrometry are shown in the Supplementary Table S1, S2. b. ASPP1/2-associated protein networks. The ASPP1/2-associated proteins are grouped by functional category (node color/label). c. Endogenous ASPP1/2 interact with multiple kinetochore components. Immunoprecipitation with anti-ASPP1 or ASPP2 antibodies were performed using cell lysates prepared from HeLa cells. The presence of kinetochore components in the immunoprecipitates was detected by WB analyses with their indicated antibodies. d. Similar to (c), the presence of three PP1 catalytic subunits in the immunoprecipitates was detected by WB analyses with the indicated antibodies.