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. 2015 Oct 19;6(39):41679–41691. doi: 10.18632/oncotarget.6149

Figure 2. TTP does not induce apoptosis of breast tumor cells.

Figure 2

Apoptosis was measured by flow cytometry in MDA-MB-231/Tet-Off cells 72 hours after withdrawing Dox in culture medium A. MCF7 cell B., TS/A C. and E0771 D. breast tumor cells were infected with control adenovirus (Ev/Ad) or TTP-expressing adenovirus (TTP/Ad) at MOI=10 for 96 hours, followed by measuring apoptosis by FACS. Caspase 3 and its cleaved products were measured by immunoblotting with anti-Caspase3 antibody in MDA-MB-231 E. and in MCF7 F. cells after TTP/Ad infection with indicated MOI. Actin serves as loading control.