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. 2015 Oct 19;6(39):41679–41691. doi: 10.18632/oncotarget.6149

Figure 4. TTP inhibits c-Jun expression at the level of transcription.

Figure 4

MDA-MB-231 A., T47D B. and MCF7 C. tumor cells were infected with TTP/adenovirus (TTP/Ad) and control adenovirus (EV/Ad). After 48 hours, total RNA was extracted to detect c-Jun expression by real time RT-PCR. c-Jun protein was detected by western blot with whole cell lysates extracted from TTP−/− (KO) and WT MEF cells D. The half-life of c-Jun mRNA was analyzed in MDA-MB-231 E. and MCF7 cells F. at 24 hours after TTP/Ad and control adenovirus (EV/Ad) infection. Total RNA was isolated as indicated time points after adding Actinomycin D (10 μg/ml), and remaining c-Jun mRNA were measured using real-time RT-PCR. The level of c-Jun mRNA before added ActD was set at 1.0. Each point is represented as mean plus SEM.