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. 2015 Oct 19;6(39):41679–41691. doi: 10.18632/oncotarget.6149

Figure 7. TTP inhibits cell proliferation through down-regulating c-Jun and up-regulating Wee1 expression.

Figure 7

MDA-MB-231 A. and MCF7 C. cells were transfected with c-Jun expression vector and empty vector for 24 h and 72 h, followed by measuring Wee1 mRNA with real time PCR. MDA-MB-231 B. and MCF7 D. cells were infected with TTP/Ad and control adenovirus (EV/Ad) for 48 h and then transfected with c-Jun expression vector or empty control vector for additional 24 h, followed by measuring Wee1 mRNA by real time PCR. For each experiment, real-time PCR assays were performed three times and each point represented by mean plus SEM. MDA-MB-231 cells were infected with TTP/adenovirus for 12 h and then transfected with c-Jun expression vector or empty vector, followed by counting cell numbers at different times as indicated E. Data shown are mean plus SD from triplicate cells of three experiments.