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. 2015 Oct 19;6(39):41750–41765. doi: 10.18632/oncotarget.6154

Figure 3. Fluorizoline induces ROS production independently of PHBs and apoptosis induction.

Figure 3

a. Jurkat cells were untreated (UT), or treated with 10 μM fluorizoline (F) for 1 h or 200 μM tert-butyl hydroperoxide (TBHP) for 30 min. b. Jurkat cells were untreated (CT) or pre-incubated with 150 μM MnTBAP for 1 h and then treated with DMSO (D) or 10 μM fluorizoline (F) for 1 h. c. Jurkat cells were untreated (CT) or pre-incubated with 150 μM MnTBAP for 1 h and then treated with DMSO (D) or 10 μM fluorizoline (F) for 24 h. Viability was measured by flow cytometry and it is expressed as the mean ± SEM (n = 3) of the percentage of non-apoptotic cells (annexin V-negative). d. Phb2fl/fl MEFs were transfected with scramble (SC) or Phb2 (siPhb2) siRNA for 72 h. Cells were then untreated or treated with 20 μM fluorizoline (F) for 1 hour. a., b., d. Superoxide anion and hydroxyl radical production was analyzed by flow cytometry using CellROX® Deep Red Reagent. Data show the mean values ± SEM relative to the mean of the control (a, n = 9; b, n = 3; d, n = 4). *p < 0.05, **p < 0.01, ***p < 0.001.