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. 2015 Oct 19;6(39):41750–41765. doi: 10.18632/oncotarget.6154

Figure 4. Fluorizoline-induced apoptosis occurs through the mitochondrial pathway in a BAX- and BAK-dependent manner.

Figure 4

a. WT, Bax−/−, Bak−/− or Bax−/−/Bak−/− MEFs were untreated (UT) or treated with 10 or 20 μM fluorizoline for 24 h. b., c. HeLa cells were transfected with scramble (SC) or BAX and BAK siRNA (siBAX/BAK) for 48 h. b. The efficiency of gene silencing was validated by western blot. β-Actin was used as a loading control. This is a representative image of at least three independent experiments. c. HeLa cells were untreated (UT) or treated with 5 or 10 μM fluorizoline (F) for 24 h. a., c. Viability was measured by flow cytometry and it is expressed as the mean ± SEM (n≥3) of the percentage of non-apoptotic cells (annexin V-negative).*p < 0.05, **p < 0.01.