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. 2015 Oct 19;6(39):41750–41765. doi: 10.18632/oncotarget.6154

Figure 6. Fluorizoline changes mRNA and protein levels of various BCL-2 family members in HeLa cells.

Figure 6

a. HeLa cells were untreated (white bars) or treated with 10 μM fluorizoline (black bars) for 24 h. mRNA levels were analyzed by RT-MLPA. Data show the mean values ± SEM of three independent experiments relative to the mean of the control. *p < 0.05, **p < 0.01, ***p < 0.001 untreated versus treated cells. b. HeLa cells were untreated or treated with 10 μM fluorizoline for 4, 8 and 24 h. c. HeLa cells were pre-incubated with 20 μM caspase inhibitor Q-VD-OPh for 30 min and then treated with 10 μM fluorizoline for 24 h. b., c. Protein levels were analyzed by western blot. Tubulin was used as a loading control. These are representative images of at least three independent experiments.