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. 2015 Oct 19;6(39):41750–41765. doi: 10.18632/oncotarget.6154

Figure 7. Loss of NOXA and BIM blocks fluorizoline-induced apoptosis.

Figure 7

a. WT, Noxa−/−, Bim−/− or Noxa−/−/Bim−/− MEFs were untreated (UT) or treated with 10, 15 or 20 μM of fluorizoline for 24 h. b., c. HeLa cells were transfected with scramble (SC), NOXA siRNA (siNOXA), BIM siRNA (siBIM), or both NOXA and BIM siRNA (siNOXA/BIM) for 48 h and then untreated (UT) or treated with 5 or 10 μM fluorizoline (F) for 24 h. b. Protein levels were analyzed by western blot. Tubulin was used as a loading control. This is a representative image of at least three independent experiments. a., c. Viability was measured by flow cytometry and it is expressed as the mean ± SEM (n ≥ 3) of the percentage of non-apoptotic cells (annexin V-negative).*p < 0.05, **p < 0.01, ***p < 0.001.