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. 2015 Oct 19;6(39):41809–41824. doi: 10.18632/oncotarget.6166

Figure 3. A B-box deletion-Cullin1 but not Cdh1 siRNA rescues the effect of FKA induced Skp2 degradation.

Figure 3

A. PC3 cells were treated with 0.1%DMSO or 20, 40 and 60 μM FKA for 24 hours. Cdh1 expression was analyzed by Western blotting. Density ratios relative to β-tubulin were shown on the top of each Western blotting band. B., C. and D. PC3 cells were transfected with vector control, siRNA controls, or Cdh1 siRNAs, or F-box deletion Skp2 (V5-del-F-box-Skp2) or B-box deletion Cullin1[flag-Dominant negative-Cullin-1, which only expression the N terminal region (1-456)]. After 24 h transfections, cells were treated with 0.1%DMSO or 40 μM FKA for 16 hours. SKp2 expression was examined by Western blotting.