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. 2015 Nov 3;6(39):42183–42196. doi: 10.18632/oncotarget.5619

Figure 2. MEK inhibitors induce apoptosis in HRAS mutant cell lines.

Figure 2

A. HRAS mutant cell lines were incubated with indicated concentrations of MEK inhibitors MEK162 for 72 hours. Then, apoptosis was determined by Annexin V and PI staining. B. Same as A. but HRAS wild-type cell lines were used instead. C. Quantification of apoptosis by AnnexinV / PI assay. Specific apoptosis was calculated for all cell lines as described in M&M section. D. Accell siRNA against HRAS (siHRAS1 and siHRAS2) were transfected in HRAS mutant and HRAS wild-type cell lines according to manufacturer's instructions. 96 hours after transfection, cells were lysed and lysates subjected to Western blot. Efficiency of knockdown was measured by anti-HRAS antibodies. Equal loading was controlled by antiactin antibodies. Cell growth was also assessed after 96 h by Cell titer Glo. Statistical significance between mutant and wild-type cell lines was calculated with student's t-test.