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Primer sequence.
| Target gene | Sequence (5′-3′) | Fragment length (bp) |
|---|---|---|
| SIRT1 | Forward: CTCCT CTCTT GACTC GCCAT | 134 |
| Reverse: CGGTG TAACC CCTCC AAGTC | ||
| GAPDH | Forward: GGGAA ACTGT GGCG TGAT | 165 |
| Reverse: AAAGG TGGAG GAGTG GGT | ||
| E-cadherin | Forward: CTTCA ATCCC ACCAC G | 184 |
| Reverse: AAATG CCATC GTTGT TC | ||
| Vimentin | Forward: TCGCC AACTA CATCG ACAAG | 339 |
| Reverse: AGGGT GTTTT CGGCT TC | ||
| N-cadherin | Forward: CCACG CCGAG CCCCA GTATC | 232 |
| Reverse: CCCCC AGTCG TTCAG GTAA TCA |
