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. 2015 Nov 14;6(38):40405–40417. doi: 10.18632/oncotarget.6334

Figure 3. Oncogenic Ras-induced macromolecule degradation requires autophagy.

Figure 3

A. Uptake of FITC-Dextran in WT MEFs and atg5 knockout (Atg5−/−) MEFs expressing KRas G12V was monitored at the indicated times. Scale bars, 20 μm. B. Uptake of TMR-BSA was also assessed in condition A.. Scale bars, 20 μm. C. HRas G12V-expressing WT MEFs and atg5−/−MEFs were each cultured in glutamine deprivation medium for 6 days, either with or without 2% BSA, and growth levels were compared by cell counting. Data are expressed relative to the values obtained for the glutamine deprivation conditions for each cell line. D. KRas G12V-expressing WT MEFs and atg5−/−MEFs were each cultured in glutamine deprivation medium for 6 days, either with or without 2% BSA, and live cell portion were measured by Annexin V and PI negative staining. Data are shown as the average of 3 independent experiments +/−SEM. Statistical significance was determined via a student's t-test.