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. 2015 Nov 17;6(38):40464–40479. doi: 10.18632/oncotarget.6342

Figure 2. Mapping of the RECQ4-MCM10 interaction domain in RECQ4.

Figure 2

A. Immunoblot analysis of two RECQ4 peptide arrays (residues 1-340; upper panel and 65-155; lower panel) after incubation of the membrane with purified GST-MCM10 and anti-MCM10 antibodies. B. Sequence alignment of RECQ4 proteins from various species in the region of RECQ4 where MCM10 binds. Identical or very similar residue groups are colored. The solid boxes show highly conserved domains and the dashed boxes less highly conserved domains. The positions of the ID1, ID2 and FL regions are indicated above. The putative SP/TP target sites for Cdk's are shown in red text. C. Immunoblot analysis using anti-RECQ4 antibodies of protein pull-down using purified GST-MCM10. The input contained either MBP-RECQ4 1-400-HIS fragment or MBP-RECQ4 1-400-HIS with the ΔFL deletion, as indicated.