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. 2015 Nov 17;6(38):40464–40479. doi: 10.18632/oncotarget.6342

Figure 3. Characterisation of the RECQ4-MCM10 interaction in human cells.

Figure 3

A. Immunoblot analysis using anti-MCM10 antibodies of protein immunoprecipitated using GFP-trap beads from U20S cells expressing YFP alone, YFP-RECQ4, YFP-RECQ4-ΔFL or YFP-RECQ4-ΔID1. The precipitated YFP proteins were stained with Ponceau S, as indicated. B. Immunoblot analysis of His-tagged RECQ4 fragments (FL or ID2) pulled-down in vitro using either GST alone or GST-MCM10. Anti-GST immunoblot was used as a loading control.