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. 2015 Nov 17;6(38):40464–40479. doi: 10.18632/oncotarget.6342

Figure 5. RECQ4-MCM10 interaction is required for normal cell cycle progression but not for RECQ4 chromatin localization.

Figure 5

A. Analysis of cell cycle transit time using flow cytometry. DT40 cells expressing YFP-RECQ, YFP-RECQ4-ΔFL or -ΔID1, as indicated, were arrested with nocadazole (NOC) and released into the next cell cycle for up to 8 hours. Points are the mean of three independent experiments. B. Western blotting analysis of cell extracts from U20S cells transfected with either of two independent siRNAs targeting RECQ4 (1 and 2) or a scrambled siRNA control (SCR). Note that the endogenous RECQ4 is depleted (top panel) but the transfected, siRNA-resistant WT RECQ4 WT and RECQ4-ΔID1, are not.