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. 2015 Oct 14;6(38):40667–40679. doi: 10.18632/oncotarget.5842

Figure 2. Effect of doxycycline on invasion, migration, and activities of matrix metalloproteinases (MMPs) of NCI-H446 and A549 cells.

Figure 2

Transwell chambers were used for the invasion assay, and images were taken at 200 × magnification. A. NCI-H446 cells were treated with 0 (b), 0.2125 (c), 0.425 (d), 0.85 (e), and 1.7 μM (f) doxycycline for 24 h. No cells were seeded in (a). Doxycycline inhibited invasion of NCI-H446 cells (P < 0.05). B. A549 cells were treated with 0 (b), 0.125 (c), 0.25 (d), 0.5 (e), and 1 μM (f) of doxycycline for 24 h. No cells were seeded in (a). Doxycycline inhibited invasion of A549 cells (P < 0.05). C. NCI-H446 cells were incubated in fetal bovine serum (FBS)-free medium containing 0, 0.2125, 0.425, 0.85, or 1.7 μM doxycycline for 24 or 48 h. Doxycycline inhibited the migration of NCI-H446 cells (P < 0.05). D. A549 cells were incubated in FBS-free medium containing 0, 0.125, 0.25, 0.5, or 1 μM doxycycline for 24 or 48 h. Doxycycline inhibited the migration of A549 cells (P < 0.05). E. MMP-2 and MMP-9 were downregulated when either cell line was treated with doxycycline. Results are expressed as percentage of control. Similar results were obtained from three independent experiments, each performed in triplicate. Results show the means of the three experiments, and the error bars represent standard deviation (*P < 0.05 and **P < 0.01).