Figure 2. UCH-L1 depletion enhances myeloma cell apoptosis in response to bortezomib or dexamethasone.

A, B. The indicated cell lines, all transduced with UCHL1 targeting doxycycline-inducible shRNAs, were incubated with or without doxycycline (DOX) and the indicated concentrations of either bortezomib (A) or dexamethasone (B) Cell viability was monitored, in triplicate, using the MTS assay. The graph represents the data from three independent experiments normalized to the viability of the cells without DOX or drug. C, D. Cells as in (A) were treated with the drugs as indicated, and the activity of caspase 3/7 was measured. The graph represents the mean +/− SEM from triplicate wells, and is representative of two independent experiments. P-values were calculated with an unpaired, two-tailed students t-test.