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. 2015 Oct 19;6(38):40775–40798. doi: 10.18632/oncotarget.5805

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Copyright: © 2015 Pu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. The growth and colony formation ability in the stable human liver cancer stem cell(HLCSC) lines and non-HLCSC transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pCMV6-A-GFP-CUDR plus pcDNA3.1-CyclinD1, pCMV6-A-GFP-CUDR plus pGFP-V-RS-PTEN, respectively. a. RT-PCR analysis of CUDR mRNA and Western bloting with anti-cyclinD1, anti-PTEN expression in stable liver cancer stem cells transfected with pCMV6-A-GFP, pCMV6-A- GFP-CUDR, pCMV6-A-CUDR plus pcDNA3.1-CyclinD1, pCMV6-A- GFP-CUDR plus pGFP-V-RS-PTEN, respectively (indicated in the left). β-actin as internalcontrol. b. Cell proliferation assay in vitro in liver cancer stem cells and unstemic liver cancer. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05. c. Cells colony-formation efficiency assay in liver cancer stem cells and unstemic liver cancer cells. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05. B. The growth and colony formation ability in the stable human liver cancer stem cell (HLCSC) lines and non-HLCSC transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pCMV6-A-GFP-CUDR plus pGFP-V-RS—CyclinD1, pCMV6-A-GFP-CUDR plus pcDNA3.1-PTEN, respectively. a. RT-PCR analysis of CUDR mRNA and Western bloting with anti-cyclinD1, anti-PTEN expression in stable liver cancer stem cells transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pCMV6-A- GFP-CUDR plus pGFP-V-RS-CyclinD1, pCMV6-A-GFP-CUDR plus pcDNA3.1-PTEN, respectively (indicated in the left). β-actin as internalcontrol. b. Cell proliferation assay in vitro in liver cancer stem cells and unstemic liver cancer cells. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05. c. Cells colony-formation efficiency assay in liver cancer stem cells and unstemic liver cancer cell. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05. C. The growth and colony formation ability in the stable human liver cancer stem cell(HLCSC) lines and non-HLCSC transfected with pGFP-V-RS, pGFP-V-RS-CUDR, pGFP-V-RS-CUDR plus pcDNA3.1-CyclinD1, pGFP-V-RS-CUDR plus pGFP-V-RS-PTEN, respectively. a. RT-PCR analysis of CUDR mRNA and Western bloting with anti-cyclinD1, anti-PTEN expression in stable liver cancer stem cells transfected with pGFP-V-RS, pGFP-V-RS-CUDR, pGFP-V-RS-CUDR plus pcDNA3.1-CyclinD1, pGFP-V-RS-CUDR plus pGFP-V-RS-PTEN, respectively (indicated in the left). β-actin as internalcontrol. b. Cell proliferation assay in vitro in liver cancer stem cells and unstemic liver cancer cells. Data are means of value from three independent experiment, bar ± SEM. **, P < 0.01; *, P < 0.05. c. Cells colony-formation efficiency assay in liver cancer stem cells and liver cancer unstemic cells. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05.

A. The growth and colony formation ability in the stable human liver cancer stem cell(HLCSC) lines and non-HLCSC transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pCMV6-A-GFP-CUDR plus pcDNA3.1-CyclinD1, pCMV6-A-GFP-CUDR plus pGFP-V-RS-PTEN, respectively. a. RT-PCR analysis of CUDR mRNA and Western bloting with anti-cyclinD1, anti-PTEN expression in stable liver cancer stem cells transfected with pCMV6-A-GFP, pCMV6-A- GFP-CUDR, pCMV6-A-CUDR plus pcDNA3.1-CyclinD1, pCMV6-A- GFP-CUDR plus pGFP-V-RS-PTEN, respectively (indicated in the left). β-actin as internalcontrol. b. Cell proliferation assay in vitro in liver cancer stem cells and unstemic liver cancer. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05. c. Cells colony-formation efficiency assay in liver cancer stem cells and unstemic liver cancer cells. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05. B. The growth and colony formation ability in the stable human liver cancer stem cell (HLCSC) lines and non-HLCSC transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pCMV6-A-GFP-CUDR plus pGFP-V-RS—CyclinD1, pCMV6-A-GFP-CUDR plus pcDNA3.1-PTEN, respectively. a. RT-PCR analysis of CUDR mRNA and Western bloting with anti-cyclinD1, anti-PTEN expression in stable liver cancer stem cells transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pCMV6-A- GFP-CUDR plus pGFP-V-RS-CyclinD1, pCMV6-A-GFP-CUDR plus pcDNA3.1-PTEN, respectively (indicated in the left). β-actin as internalcontrol. b. Cell proliferation assay in vitro in liver cancer stem cells and unstemic liver cancer cells. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05. c. Cells colony-formation efficiency assay in liver cancer stem cells and unstemic liver cancer cell. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05. C. The growth and colony formation ability in the stable human liver cancer stem cell(HLCSC) lines and non-HLCSC transfected with pGFP-V-RS, pGFP-V-RS-CUDR, pGFP-V-RS-CUDR plus pcDNA3.1-CyclinD1, pGFP-V-RS-CUDR plus pGFP-V-RS-PTEN, respectively. a. RT-PCR analysis of CUDR mRNA and Western bloting with anti-cyclinD1, anti-PTEN expression in stable liver cancer stem cells transfected with pGFP-V-RS, pGFP-V-RS-CUDR, pGFP-V-RS-CUDR plus pcDNA3.1-CyclinD1, pGFP-V-RS-CUDR plus pGFP-V-RS-PTEN, respectively (indicated in the left). β-actin as internalcontrol. b. Cell proliferation assay in vitro in liver cancer stem cells and unstemic liver cancer cells. Data are means of value from three independent experiment, bar ± SEM. **, P < 0.01; *, P < 0.05. c. Cells colony-formation efficiency assay in liver cancer stem cells and liver cancer unstemic cells. Data are means of value from three independent experiment, bar ± SEM. ** P < 0.01; * P < 0.05.