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. 2015 Oct 20;6(38):40799–40814. doi: 10.18632/oncotarget.5695

Figure 4. Activation of AKTs by miR-29b silencing contributes to the activation of key enzymes in the Warburg effect in ovarian cancer cells.

Figure 4

A. A schematic is provided to demonstrate various enzymes involved in regulating glycolytic metabolism of cancer cells; B. Correlation analysis between speed-limiting glycolytic genes (LDHA, PKM2, GLUT1, and HK2) expressions and miR-29b levels using the NCI-60 expression profiling data; C. and D. qPCR and WB results show changes in AKT2, AKT3 and four interested glycolytic enzymes when miR-29b is silenced by target inhibitors transfection in A2780 and 3AO cells at mRNA and protein levels, respectively; E. and F. Key glycolytic product pyruvic acid was increased by miR-29b silencing, while the ratio of NAD+/NADH decreased as ovarian cancer cells were transfected with miR-29b inhibitors in both A2780 and SKOV3 cells. Data are presented as means ± SEM. *p < 0.05 versus control.