Table 1. Primers list.
Genes | Primer Sequence (5′-3′) | Experimental use |
---|---|---|
AKT2 | F: GAGGTCATGGAGCACAGGTT R: CTGGTCCAGCTCCAGTAAGC |
Real-time PCR |
AKT3 | F: GGCGAGCTGTTTTTCCATTTG R: GGCCATCTTTGTCCAGCATTAG |
Real-time PCR |
HK2 | F: TGTGCGTAATGGCAAGCGGAGG R: CCACGGCAACCACATCCAGGTC |
Real-time PCR |
PKM2 | F: CCACTTGCAATTATTTGAGGAA R: GTGAGCAGACCTGCCAGACT |
Real-time PCR |
beta-actin | F:TCCCTGGAGAAGAGCTACGA R:AGCACTGTGTTGGCGTACAG |
Real-time PCR |
miR-29b | Mature chain: UAGCACCAUUUGAAAUCAGUGUU RT primer: CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAACACTGA F: ACACTCCAGCTGGGTAGCACCATTTGAAATC R: TGGTGTCGTGGAGTCG |
Real-time PCR |
U6 | RT primer: AACGCTTCACGAATTTGCGT F: CTCGCTTCGGCAGCACA R: TGGTGTCGTGGAGTCG |
Real-time PCR |