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. 2015 Oct 12;6(38):40896–40906. doi: 10.18632/oncotarget.5683

Figure 4. A. Prolonged passage after IDH1-R132H expression increased chromatin deposition of H3K27me3 in human U87MG glioblastoma and murine Ink4a/Arf−/− astrocytic cells.

Figure 4

Cell lysates from short (5 passages) and long-term passaged (28 passages) cells were prepared, fractionated by gel-electrophoresis, and probed with an anti-H3K27me3 (Abcam#6002), anti-Flag (Sigma#8592), or anti-Ku86 (Santa Cruz#sc-1485) antibody. Right: Densitometry quantitation of the immuno-blot. The H3K27me3 signal was normalized to the Ku86 signal. B. Prolonged passage after IDH1-R132H expression in human U87 glioblastoma cells induced altered DNA methylation patterns from a G-CIMP- pattern to a G-CIMP+ pattern. Red indicates that the genomic region of interest is methylated. Green indicates the lack of DNA methylation. C. Prolonged passage after IDH1-R132H expression increased TMZ resistance of human U87MG glioblastoma and murine Ink4a/Arf−/− astrocytic cells. Clonogenic survival was assessed 14 days after TMZ treatment. Please note that the Y-axis is plotted on a log-scale.