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. 2015 Oct 1;6(38):40970–40980. doi: 10.18632/oncotarget.5403

Figure 3. Biotin-labeled hGal-7(129–135) is capable of binding to recombinant hGal-7.

Figure 3

Binding curve showing a dose-dependent interaction between biotin-labeled hGal-7(129–135) and hGal-7 or hGal-1. Recombinant hGal-7 or hGal-1 (10 μg/ml) were coated on 96-well plates overnight and then incubated 60 min with unlabeled hGal-7(129–135) (1 mM) to eliminate non-specific binding. Incubation with increasing concentrations of biotin-labeled hGal-7(129–135) was performed for 120 min. Results are representative of three independent experiments. Error bars represent standard deviation.