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. 2015 Oct 16;6(38):41018–41032. doi: 10.18632/oncotarget.5879

Figure 5. Fatostatin synergizes with docetaxel to inhibit cell proliferation and induce apoptosis in PCa cells harboring different p53 status.

Figure 5

A. The combination of fatostatin and docetaxel suppressed cell viability in PC-3 EV, PC-3 R248W and DU145 cells. Fato, fatostatin; Doce, docetaxel; Comb, combination. The combination index (CI) was calculated by Calcusyn software and is shown at the bottom. B. The combination of fatostatin (2.5 μM) and docetaxel (5.0 nM) significantly decreased colony formation by PC-3 EV, PC-3 R248W and DU145 cells. The number of colonies was counted and reported as the mean ± SD of triplicate experiments. **P < 0.01, ***P < 0.001. Con, control. C. The combination treatment significantly increased caspase-3/7 activity of PC-3 EV, PC-3 R248W and DU145 cells. Data are plotted as the relative units of luciferase intensity and reported as the means ± SD values from triplicate experiments. **P < 0.01, ***P < 0.001. D. Western blot analysis of apoptosis-related markers (caspase-3 and PARP) in PC-3 EV, PC-3 R248W and DU145 cells treated with fatostatin, docetaxel, alone or in combination for 72 hours. GAPDH was used as a loading control.