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. 2015 Oct 15;6(38):41045–41055. doi: 10.18632/oncotarget.5728

Figure 1. MALAT1 binds to EZH2 in PCa cells.

Figure 1

A. RIP-seq profile showing the pulldown of MALAT1 by EZH2. Screen shots of the UCSC genome browser showing coverage profiles of MALAT1 in EZH2 RIP-seq and input samples. Both RIP-seq and input samples were normalized into the same sequencing depth. B–D. LNCaP-Rf and C4-2 CRPC cells were harvested for RIP with anti-EZH2 antibody or control IgG. Bound MALAT1 (B, C) and FOXO1 mRNA (negative control) (D) were analyzed by RT-qPCR (n = 3). Data are means ± S.D. from experiments with three replicates. *P < 0.01 comparing to the IgG control group. n.s., not statistically significant. E. RT-qPCR analysis of MALAT1 in RIP samples pulled down by anti-EZH2 antibody in LNCaP androgen-sensitive cells. *P < 0.01 comparing to the IgG control group.