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. 2015 Oct 15;6(38):41045–41055. doi: 10.18632/oncotarget.5728

Figure 3. The effect of MALAT1 on expression of Polycomb-dependent target genes of EZH2.

Figure 3

A–D. PC-3 (A, C) and C4-2 (B, D) cells were transfected with non-specific control (siC) or two individual MALAT1-specific siRNAs (siM-1 and siM-2) for 48 h and then cells were harvested for RT-qPCR analysis of MALAT1 (A, B) and EZH2 Polycomb-dependent target genes DAB2IP and BRACHYURY (C, D). Data are means ± S.D. from experiments with three replicates. *P < 0.01 comparing to the siC group. E. PC-3 and C4-2 cells were transfected as in (A) and expression of DAB2IP proteins were analyzed by western blot. ERK2 was used as a loading control.