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. 2015 Oct 15;6(38):41045–41055. doi: 10.18632/oncotarget.5728

Figure 4. The effect of MALAT1 on EZH2 recruitment and H3K27me3 levels at EZH2 Polycomb-dependent target gene loci.

Figure 4

A, B. ChIP-qPCR analysis of EZH2 occupancy at its target loci DAB2IP and BRACHYURY. PC-3 (A) and C4-2 (B) cells were transfected with non-specific control (siC) or a pool of MALAT1-specific siRNAs (siMALAT1). 48 h after transfection, cells were harvested for ChIP assay with non-specific IgG or EZH2 antibody. Data are means ± S.D. from experiments with three replicates. *P < 0.01. C, D. ChIP-qPCR analysis of H3K27me3 levels at the promoters of EZH2 target loci DAB2IP and BRACHYURY. PC-3 (C) and C4-2 (D) cells were transfected with non-specific control (siC) or a pool of MALAT1-specific siRNAs. 48 h after transfection, cells were harvested for ChIP assay with non-specific IgG or H3K27me3 antibody. Data are means ± S.D. from experiments with three replicates. *P < 0.01.