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. 2015 Oct 15;6(38):41045–41055. doi: 10.18632/oncotarget.5728

Figure 6. MALAT1 expression correlates with EZH2 mRNA levels and MALAT1 enhances expression of PRC2-independent target genes of EZH2 in CRPC cells.

Figure 6

A. Meta-analysis of correlation between MALAT1 and EZH2 mRNA expression in human primary and castration-resistant PCa specimens. Pearson correlation test r = 0.5, P = 4.3E-7. B. RT-qPCR analysis of expression of MALAT1 and EZH2 mRNA in 12 cases of human CRPC specimens. Pearson correlation test r = 0.57, P = 0.026. C. RT-qPCR analysis of MALAT1, CKS2 and TMEM48 in C4-2 cells at 48 h after transfected with control siRNAs (siC) or MALAT1-specific siRNAs (siM-1 and siM-2). Data are means ± S.D. from experiments with three replicates. *P < 0.01 comparing to the siC group. D. The effect of MALAT1 on TMEM48 expression is mediated through EZH2. C4-2 cells were transfected with non-specific control (siC) or MALAT1- and/or EZH2-specific siRNA and at 48 h after transfection cells were harvested for RNA isolation and RT-qPCR analysis of expression of TMEM48. Data are means ± S.D. from experiments with three replicates. *P < 0.01. E. mRNA expression of MALAT1, EZH2 and DAB2IP in androgen-dependent (LuCaP 23.1) and castration-resistant (LuCaP 23.1AI) xenograft tumors analyzed by RT-qPCR. Data are means ± S.D. from experiments with three replicates. *P < 0.01 comparing the data in 23.1AI with those in 23.1 tumors. F. RT-qPCR analysis of TMEM48 and KIAA0101 expression in LuCaP 23.1 and LuCaP 23.1AI xenograft tumors. Data are means ± S.D. from experiments with three replicates. *P < 0.01.