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. 2015 Oct 12;6(38):41077–41091. doi: 10.18632/oncotarget.6218

Figure 5. UBE2L6 induces apoptosis in NPC cells.

Figure 5

A. CNE2 cells were transfected with empty vector or UBE2L6 expression vector for 24 hrs. Apoptosis were then assessed by flow cytometry after double staining with Annexin V-FITC and PI. Cells that were positively stained by Annexin V-FITC only (early apoptosis) and positive for both Annexin V-FITC and PI (late apoptosis) were quantitated, and both subpopulations were considered as apoptotic cells (mean ± s.d, n = 3). B. At 72 hrs after transfection in CNE2 cells with either empty vector or UBE2L6, the Caspase-3 enzyme activity was measured with Ac-DEVD-pNA by a colorimetric assay ((mean ± s.d, n = 3). C. Cleaved Caspase-3 protein was detected by western blot in transfected CNE2 cells. The arrows point to cleaved Caspase-3 fragments with molecular weights of 17kDa and 19kDa. *: p < 0.05.