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. 2015 Oct 14;6(38):41123–41133. doi: 10.18632/oncotarget.5660

Figure 6. The apoptotic efficacy of lapatinib in combination with TORC1 or TORC2 targeting.

Figure 6

A. SKBr3 cells stably expressing dox-inducible inactivation of TORC1 (by Raptor shRNA) or TORC2 (Rictor shRNA) were treated with or without 200 ng/ml doxycycline, and upon knockdown of Raptor or Rictor, were also treated for an additional 72 hours with or without lapatinib. The timepoints of Rictor and Raptor knockdown were previously determined in pilot experiments. After 72 hours of lapatinib treatment, apoptotic fraction was assayed by FACS analysis of DNA degradation. The data shown is the mean of triplicates and error bars represent S.E.M. The P values for the comparisons indicated by the stars is shown to the right of the stars. B. The identical experiment is shown for BT474 cells.