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. 2015 Oct 15;6(38):41324–41338. doi: 10.18632/oncotarget.5847

Figure 6. DR5 suppression-induced promotion of cell invasion requires FADD, caspase-8 (A) and TRAF2 (E), but not caspase-8 activity (B–D).

Figure 6

A and E. A549 cells transfected with the indicated siRNAs alone or in combinations were seeded in 12-well plates for Western blotting to detect the given proteins, in Matrigel invasion chambers for cell invasion assays and in 96-well plates for cell growth measurements after approximately 48 h incubation. The data are means ± SDs of triplicate (A) or duplicate (E) determinations (invasion) or quadruplicate determinations (cell growth). B and C, A549 (B) and HCT116 DR5-KO (C) cells transfected with control (Ctrl) or indicated siRNA were plated in Matrigel invasion chambers for approximately 12 h and then exposed to the given caspase inhibitors (20 μM) in the bottom wells for an additional 36 h. The invasive cells were stained and measured. In addition, the cells were also plated in 96-well plates and received the same treatments with the indicated caspase inhibitors for 48 h for measurement of cell growth with the MTS assay. The data are means ± SDs of triplicate (invasion) or quadruplicate (cell growth) determinations. D, HCT116 cells were seeded in 96-well plates and treated with 50 ng/ml TRAIL alone or in combination with 20 μM Z-VAD or Z-IETD. After 4 h, the cell viability was measured with the MTS assay. The data are means ± SDs of quadruplicate determinations.