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. 2015 Oct 21;6(38):41383–41397. doi: 10.18632/oncotarget.5510

Figure 1. SPC24 mRNA expression in HCC specimens analyzed by RT-PCR and real-time RT-PCR.

Figure 1

A. The mRNA of SPC24 was measured in 20 cases of HCC specimens [C] and corresponding adjacent noncancerous liver tissues [N] by RT-PCR. The relative mRNA level of SPC24 was normalized based on that of an internal reference β-actin. PCR products were visualized by electrophoresis on 2% agarose gels. B. The mRNA of SPC24 was evaluated in 9 cases of normal liver tissues by semi-quantitative RT-PCR. β-Actin was used as an internal control. PCR products were visualized after electrophoresis through 2% agarose. C. The mRNA levels of SPC24 in 212 paired HCC specimens and adjacent noncancerous liver tissues (ANLT) were determined by quantitative real-time PCR, β-Actin was used as an internal control. D. Combined analysis of SPC24 mRNA expression and AFP level in examined 212 cases of HCC. The number represents the percentage among the 212 cases of HCC patients.