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. 2016 Feb 9;11(2):e0147334. doi: 10.1371/journal.pone.0147334

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© 2016 Malanga et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — A. PCR analysis of lung DNA from R26-AKT1^E17K; Ttf1-Cre and control littermates. ∆Stop tpA: lox-P flanked transcription termination stop signal. B. Relative mRNA expression of human AKT1 by Q-RT-PCR on RNA of lungs from R26; Ttf1-Cre, R26-AKT1^E17K; Ttf1-Cre. Data are presented from replicate analysis as the mean±SD. C. Representative immunoblot analysis of pAKT, total AKT1 and downstream signalling proteins in total protein extracts from whole lungs of R26; Ttf1-Cre and R26-AKT1^E17K; Ttf1-Cre mice, respectively. D-E. Representative H&E staining of lungs from R26;Ttf1-Cre and R26-AKT1^E17K; Ttf1-Cre, respectively. Magnification as indicated. F-G. Representative pAKT staining of lungs from R26;Nkx2.1-Cre and R26-AKT1^E17K;Nkx2.1-Cre, respectively. Magnification as indicated.