Table 1. Evaluation of hyperplastic lesions in R26-AKT1E17K mice.
Hyperplastic lesions were classified on the basis of the number of epithelial layers and the percentage of hyperplasia. Evaluation of epithelial layers, score 1: 1–2 layers of epithelial cells, score 2: 2–3 layers of epithelial cells, score 3: >4 layers of epithelial cells (n = 6 mice/group; 3 serial sections/mouse). Evaluation of the extent of hyperplasia, score 1: hyperplasia in 1–20% of total sections analysed; score 2: hyperplasia in 21–60% of total sections analysed; score 3: hyperplasia in 61–100% of total sections analysed (n = 6 mice/group; 3 serial sections/mouse). Lesions were classified according to a Combined score resulting from the sum of the score of layers and score of the percentage of hyperplasia observed (p<0.05; t-Student).
| Mouse code | Treatment | Number of Epithelial Layers (score) | Percentage of hyperplasia (score) | Combined score | Mean of Final scores |
|---|---|---|---|---|---|
| #1a | Vehicle | 1 | 1 | 2 | 2.5±0.3 |
| #2a | Vehicle | 2 | 1 | 3 | |
| #3a | Vehicle | 1 | 1 | 2 | |
| #4a | Vehicle | 1 | 1 | 2 | |
| #5a | Vehicle | 2 | 2 | 4 | |
| #6a | Vehicle | 1 | 1 | 2 | |
| #7a | Ad-CRE | 2 | 3 | 5 | 3.8±0.4 |
| #8a | Ad-CRE | 1 | 1 | 2 | |
| #9a | Ad-CRE | 2 | 2 | 4 | |
| #10a | Ad-CRE | 2 | 2 | 4 | |
| #11a | Ad-CRE | 2 | 1 | 3 | |
| #12a | Ad-CRE | 2 | 3 | 5 |