Table 1. Primers used for plasmids construction.
| Primers | Primer sequence | Genes |
|---|---|---|
| mZVF | 5' ATC GTC CAA TGC TAA AAA GGG CC CAT GGT CGG GTT CC 3' | V protein of ZJ1 |
| mZVR | 5' AAC CCG ACC ATG GGCCC TTT TTA GCA TTG GAC GAT 3' | |
| mLVF | 5' CAA TGC TAA AAA GGG CC CAT GGT CGA GCC CCC AAG AG 3' | V protein of LaSota |
| mLVR | 5' TTG GGG GCT CGA CCA TGG GC CCT TTT TAG CAT TGG A 3' | |
| m9VF | 5' ATC GAA TGC TAA AAA GGG CC CAC CTC AGA GCC CTC CA 3' | V protein of 9a5b |
| m9VR | 5' GAG GGC TCT GAG GTG GGCCC TTT TTA GCA TTC GAT 3' | |
| p9PNF | 5' GGT GAA TTC ATG GCT ACG TTC ACG GAC GCA GAG A 3' | V protein of 9a5b |
| p9VCR | 5' CAA TCC GAG TCA ATC TTG CTT ACC CTC TGT G 3' | |
| ZJVCDF | 5' A TCA GAA TTC ATG GTC GGG TTC CCA AGA AGG GCA TCA CCA AC 3' | C-terminal domain of ZJ1 V |
| pZJVR | 5' CTG ATG A GTC GAC TTA CTT ACC TTC TGT GAT AAT GCC TCC A 3' | |
| Fla14sta1F | 5' AGGGATATCGATGTCTCAGTGGTACGAACTTCAGCAGCT 3' | Human STAT1α and its mutant |
| Fla14sta1R | 5' TCATGGATCCTACTGTGTTCATCATACTGTCGAATTCTA 3' |
Note: To construct V ORFs, an extra one G was inserted behind the RNA-editing site of pCI-P/ZJ1, pCI-P/LaSota and pCI-P/9a5b using primer sets mZVF/mZVR, mLVF/mLVR and m9VF/m9VR. The added extra G was boxed. To produce anti-V mouse polyclonal serum, the complete V ORF and CTD sequence were PCR-amplified from pCI-V/9a5b and pCI-V/ZJ1 constructed with primer sets p9PNF/ p9VCR and ZJVCDF/ pZJVR. Fla14sta1F/Fla14sta1R were used for PCR amplification of the complete ORFs of wt or mutated human STAT1 protein from plasmids stat1 alphap RC/CMV and stat1 alphap Y701F RC/CMV. The restriction endonuclease sites were underlined.