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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Arch Biochem Biophys. 2015 Dec 11;591:111–131. doi: 10.1016/j.abb.2015.11.005

Figure 7. Microarray and miRNA array from endothelial RNA in PCL model identifies novel mechanosensitive miRNAs and genes.

Figure 7

Following partial carotid ligation, endothelial RNA was collected either 12 or 48 hours after ligation and subject to a microarray. Both scatter plots of the normalized intensities of each probe and heat maps of single samples pooled from 3 different LCAs or RCAs show the number of genes affected by flow increase from 12 to 48 hours. The Venn diagrams also show the temporal effects of d-flow on the number of up- or down-regulated mechanosensitive genes Adapted from Ni et al. (2010) (A). Endothelial RNA collected 48 hours post-ligation (pooled from three mice) was also analyzed by miRNA array. The heat map shows several miRNAs which are differentially regulated by flow, several of which were validated by qPCR (data shown as mean±s.e.m; *P<0.05 as determined by paired t-test). Adapted from Son et al. (2013) (B).