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. Author manuscript; available in PMC: 2017 Feb 12.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Jan 23;470(3):606–612. doi: 10.1016/j.bbrc.2016.01.097

Figure 1. Adiponectin inhibits Wnt/β-catenin signaling.

Figure 1

Primary human skin fibroblasts were grown to confluency and serum-starved overnight. Cells were then treated with recombinant adiponectin (APN; 1 or 5 µg/ml). Twenty-four hours later, cells were treated with Wnt3a (100ng/ml). After 24 hours, RNA was isolated and qPCR was conducted on β-catenin target genes, Axin2 (A) and Naked1 (B). Graphs represent means from independent experiments and error bars represent standard deviation (SD) (n = 4 or 3 in A or B, respectively). C&D. Twenty-four hours after Wnt3a treatment, lysates were collected and GST-ICAT pull-down assays were used to measure levels of cadherin-free β-catenin. The graph in D shows relative levels of cadherin-free β-catenin normalized to total β-catenin from 4 independent experiments. Panel C shows representative blot from those graphed in D. Asterisks * and ** represent statistical significance by Student’s t-test, p<0.05 and <0.01, respectively).