Table S2.

Contour length gains of the dimer opening event

Unstructured part C domains (amino acid region)	Distance from crystal structure, nm	Expected length gain, nm	Measured length gain,* nm
Residues 675–715 (C terminal helix stable)	0.8	29.1	40.56 ± 1.06
Residues 656–715 (C terminal helix unstable)	1.7	41.4	40.56 ± 1.06

Comparison of the measured with the expected contour length gain of the dimer opening event for two scenarios. In the first, the last C-terminal alpha helix is considered to be stable; in the second, it is considered unstable in the not dimerized state. For a discussion, see SI Structure Sizes from WLC Fits.

^*Errors are SD.