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. 2015 Dec 22;113(5):E509–E518. doi: 10.1073/pnas.1512952113

Fig. 2.

Fig. 2.

The effects of notch ectodomain length on its interaction with γ-secretase. (A) Schematic diagram of notch-based substrates. N43 is a synthetic peptide, whereas the other substrates were produced recombinantly. (B) Kinetic analysis of notch with varying ectodomain length (mean ± SD, n = 3) and (C) of N43 substrate (mean ± SD, n = 3). (D) Comparing the ability of γ-secretase to immunoprecipitate in complex with V1711 and Ub1711. γ-secretase was immunoprecipitated via its HA tag on Aph-1. Substrates were visualized by their C-terminal myc tag. (E) Summary of kinetic data from B and C (mean ± SD, n = 3; ND, not determined).