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. 2015 Dec 22;113(5):E509–E518. doi: 10.1073/pnas.1512952113

Fig. 5.

Fig. 5.

The effects of chemical reduction of disulfides on nicastrin structure. (A) Circular dichroism spectrum of purified nicastrin ectodomain with and without TCEP present. (B) Limited proteolysis of pure nicastrin ectodomain with varying amounts of trypsin in the presence and absence of DTT. (C) Quantification of intact nicastrin ectodomain from B (mean ± SD, n = 2). (D) Limited proteolysis of nicastrin in the γ-secretase complex with varying amounts of proteinase K and thermolysin proteases.