Fig. S3.
Estimation of the binding affinity between γ-secretase and substrate. (A) γ-secretase concentrations ranging from 0.625–10 nM were incubated with 20 nM V1711 before co-IP via the HA tag on Aph-1. (B) Myc-tagged V1711 concentrations ranging from 1.25–20 nM were incubated with 5 nM γ-secretase before co-IP via the HA tag on Aph-1. (C) A total of 5 nM γ-secretase was incubated with 20 nM V1711 before pull-down of the complex via the myc tag of V1711 with anti-myc affinity resin. Quantification reveals apparent Kd values averaging in the mid-nM range.