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Analysis of products resulting from the methylation of HeLaNuc catalyzed by NSD2. (A) A diagram showing the H3 peptide containing Lys27-Arg40 resulting from ArgC digestion of HeLaNuc that were methylated by NSD2 in vitro. (B) LC-MS analysis of the products resulting from methylation of HeLaNuc showed only monomethylation and dimethylation of H3K36 for starting peptides containing between zero and three preexisting methyl marks.
