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shRNA-mediated knockdown efficiency of MAFG, BACH1, CHD8, and DNMT3B in SK-MEL-28 cells. qRT-PCR (A) and immunoblot (B) analysis monitoring shRNA-mediated knockdown efficiency for MAFG, BACH1, CHD8, and DNMT3B in SK-MEL-28 cells using two unrelated shRNAs against the same target gene. Data are represented as mean ± SD. *P ≤ 0.05; **P ≤ 0.01. NS, nonsilencing.
