Table S3.
Drosophila strains and transgenic lines
| Fly strain | Source | Details |
| Wild-type, balancer and mutant flies | ||
| White Dahomey wolbachia plus (wDah w+) | 12 | Wild-type Drosophila stock |
| wDah w+; CyO | Bloomington Drosophila Stock Center | Balancer fly on the second chromosome, homozygous lethal, Curly wings |
| wDah w+;; TM3Sb | Bloomington Drosophila Stock Center | Balancer fly on the third Chromosome, homozygous lethal |
| wDah w+; chico1 | 13 | A Drosophila insulin receptor substrate protein |
| wDah w+;; DHR96Δ | 1 | DHR96 null mutation on the third chromosome |
| GAL4 driver lines | ||
| wDah w+;; mhc-GAL4 | Bloomington Drosophila Stock Center | Muscle-specific driver, chromosome 3 |
| wDah w+;; mhc-Gal4/ DHR96 Δ | This study | Muscle-specific driver in a DHR96 null background |
| wDah w+; dilp2-GAL4 | 14 | mNSC-specific driver (median neurosecretory cell) |
| wDah w+;; InsP3-GAL4 | 15 | mNSC-specific driver (median neurosecretory cell) |
| wDah w+;; InsP3-GAL4/DHR96 Δ | This study | mNSC-specific driver (median neurosecretory cell) in a DHR96 null background |
| wDah w+; Uo-GAL4 | 16 | Malpighian Tubule-specific driver |
| UAS-responder lines | ||
| wDah w+; UAS-Cyp6g1-8a | 17 | Cytochrome P450 6g1 |
| wDah w+; UAS- DHR96-WT | This study | Wild type UAS-DHR96 line on chromosome 2 |
| wDah w+; UAS-dFOXO | 18 | dFOXO inserted through attp40 sites into the second Chromosome |
| wDah w+; UAS-dFOXO; DHR96Δ | This study | UAS-dFOXO in a DHR96 null background |
| wDah w+, UAS-rpr | 14 | UAS-reaper |
| wDah w+, UAS-rpr;; DHR96Δ | This study | UAS-reaper in a DHR96 null background |