Fig. S6.
IFN response after transfection with EBER expression plasmids or in vitro-transcribed 5′pppEBER1. (A) IFN-β promoter activation (assessed with a luciferase-reporter construct) after transfection with EBER plasmid (1 EBER copy) or EBERhigh plasmid (10 EBER copies) in Hek293 cells expressing TLR3, TLR7, or RIG-I. Reporter firefly luciferase activity is normalized to Gaussia luciferase activity and data are expressed as fold-change relative to the mock control. (B) Detection of EBER1 and EBER2 in Hek293 cells transfected with EBER expression plasmids (Upper) and endogenous EBER1 and EBER2 levels in LCL cells and exosomes (Lower). (C and D) IFITM1 and IFITM3 expression in LCLs at indicated time points after transfection with in vitro transcribed 5′pppEBER1. (E) qPCR analysis of IFIT1, IFIT3, and IFIT5 in DCs after treatment with LCL or BJAB exosomes.