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. 2016 Jan 14;113(5):E587–E596. doi: 10.1073/pnas.1518130113

Table S1.

List of adapter, primers, and probes

Target Oligonucleotide Sequence (5′- 3′)
Small noncoding RNA
 EBER1 Stem-loop RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAACATGCG
Forward AGGACCTACGCTGCCCTAGA
Stem-loop Reverse ATTCAGTTGAGAAAACATGCGG
 EBER2 Stem-loop RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAATAGCG
Forward AGGACAGCCGTTGCCCTAGTGGTTTC
Stem-loop Reverse ATTCAGTTGAGAAAAATAGCGG
 vtRNA1-1 Stem-loop RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAGGACTG
Forward GGCTGGCTTTAGCTCAGCGG
Stem-loop Reverse ATTCAGTTGAGAAAAGGACTGG
 RNY1 Stem-loop RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAGACTAG
Forward GCTGGTCCGAAGGTAGTGAG
Stem-loop Reverse ATTCAGTTGAGAAAAGACTAGT
 5′-adapter-EBER1 RA5 adapter GUUCAGAGUUCUACAGUCCGACGAUC (Illumina #15013205)
Forward (adapter) GTTCAGAGTTCTACAGTCCGA
Reverse (EBER1) AAAACATGCGGACCACC
miRNA
 EBV BART1-5p Stem-loop RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACAGCACG
Forward ACACTCCAGCTGGGTCTTAGTGGAAGTGAC
Probe TTCAGTTGAGACAGCACG
Reverse CTCAACTGGTGTCGTGGAGTCGGCA
 EBV BART8-3p Stem-loop RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCTACGACC
Forward ACACTCCAGCTGGGGTCACAATCTATGGGG
Probe TTCAGTTGAG CTACGACC
Reverse CTCAACTGGTGTCGTGGAGTCGGCA
 EBV BART17-5p Stem-loop RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCTTGTATG
Forward ACACTCCAGCTGGGTAAGAGGACGCAGGCA
Probe TTCAGTTGAGCTTGTATG
Reverse CTCAACTGGTGTCGTGGAGTCGGCA
 EBV BHRF1-2* Stem-loop RT CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGCTATCTG
Forward ACACTCCAGCTGGGAAATTCTGTTGCAGCA
Probe TTCAGTTGAGGCTATCTG
Reverse CTCAACTGGTGTCGTGGAGTCGGCA
 hsa-miR21-5p TaqMan MicroRNA Assay ID 000397
 hsa-miR451 TaqMan MicroRNA Assay ID 001105
mRNA
 IFN-β Forward CCTGAAGGCCAAGGAGTACA
Reverse AAGCAATTGTCCAGTCCCAG
 IFITM1 Forward ATGTCGTCTGGTCCCTGTTC
Reverse GTCATCAGGATGCCCAGAAT
 IFITM3 Forward ATGTCGTCTGGTCCCTGTTC
Reverse GGGATGACGATGAGCAGAAT
 OAS2 Forward GGTGAACACCATCTGTGACG
Reverse ACCATCGGAGTTGCCTCTTA
 MX1 Forward GATTTTGGGGCTTTCCAGTC
Reverse GATGATCAAAGGGATGTGGC
 TLR3 Forward TCACTTGCTCATTCTCCCTT
Reverse GACCTCTCCATTCCTGGC
 TLR7 Forward TGCTCTGCTCTCTTCAACCA
Reverse AACCATCTAGCCCCAAGGAG
 TLR9 Forward ACACAGCTGCGCAAGCTTAAC
Reverse TCGAGTGAGCGGAAGAAGATG
 PKR Forward GGGACCTTGGAACAATGGAT
Reverse CCTTGTTCGCTTTCCATCAT
 RIG-I Forward ATATCCGGAAGACCCTGGAC
Reverse GAGAAAAAGTGTGGCAGCCT
 IFIT1 Forward TACCTGGACAAGGTGGAGAA
Reverse GTGAGGACATGTTGGCTAGA
 IFIT3 Forward CTTACGGCAAGCTGAAGAGT
Reverse AATTGCCAGTCCAGAGGAGA
 IFIT5 Forward GGCCATTCTGTTGGAGTTAG
Reverse GCTTCTTCAAGCTGGTCCAT
 MVP Forward ATCTGAGGCGTTTGTTGCAG
Reverse GGATGCGGATGATGAACTCT
 La/SSB Forward TAATGAAAAGATGGCTGCCC
Reverse AAGGTACCCAGCCTTCATCC
 TIM1 Forward CCAGTAGCCACTTCACCATCTTCAC
Reverse CGGTGTCATTCCCATCTGTTGTG
 TIM4 Forward ACAGGACAGATGGATGGAATACCC
Reverse AGCCTTGTGTGTTTCTGCG
GAPDH Forward GAGTCAACGGATTTGGTCGTAT
Reverse ATGGGTGGAATCATATTGGAAC
EBV DNA
 EBNA1 QK Forward GCCGGTGTGTTCGTATATGG
Reverse CAAAACCTCAGCAAATATATGAG
Probe 1 TCTCCCCTTTGGAATGGCCCTG-fluorescein
Probe 2 LCRed640-ACCGGCCCACAACCTG
Cloning
 EBER1 Forward TGCAAGATCTTAATACGACTCACTATAGGAGGACCTACGCTGCCATAGA
Short Forward* TGCAAGATCTTAATACGACTCACTATAGGAGGACC
Reverse ACGTTCTAGAAAACATGCGGACCACCAGC

The T7 promoter sequence is underlined.

*

Added after 20 rounds of amplification to increase PCR efficiency and yield.