Table S1.
Target | Oligonucleotide | Sequence (5′- 3′) |
Small noncoding RNA | ||
EBER1 | Stem-loop RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAACATGCG |
Forward | AGGACCTACGCTGCCCTAGA | |
Stem-loop Reverse | ATTCAGTTGAGAAAACATGCGG | |
EBER2 | Stem-loop RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAATAGCG |
Forward | AGGACAGCCGTTGCCCTAGTGGTTTC | |
Stem-loop Reverse | ATTCAGTTGAGAAAAATAGCGG | |
vtRNA1-1 | Stem-loop RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAGGACTG |
Forward | GGCTGGCTTTAGCTCAGCGG | |
Stem-loop Reverse | ATTCAGTTGAGAAAAGGACTGG | |
RNY1 | Stem-loop RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGAAAAGACTAG |
Forward | GCTGGTCCGAAGGTAGTGAG | |
Stem-loop Reverse | ATTCAGTTGAGAAAAGACTAGT | |
5′-adapter-EBER1 | RA5 adapter | GUUCAGAGUUCUACAGUCCGACGAUC (Illumina #15013205) |
Forward (adapter) | GTTCAGAGTTCTACAGTCCGA | |
Reverse (EBER1) | AAAACATGCGGACCACC | |
miRNA | ||
EBV BART1-5p | Stem-loop RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGACAGCACG |
Forward | ACACTCCAGCTGGGTCTTAGTGGAAGTGAC | |
Probe | TTCAGTTGAGACAGCACG | |
Reverse | CTCAACTGGTGTCGTGGAGTCGGCA | |
EBV BART8-3p | Stem-loop RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCTACGACC |
Forward | ACACTCCAGCTGGGGTCACAATCTATGGGG | |
Probe | TTCAGTTGAG CTACGACC | |
Reverse | CTCAACTGGTGTCGTGGAGTCGGCA | |
EBV BART17-5p | Stem-loop RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCTTGTATG |
Forward | ACACTCCAGCTGGGTAAGAGGACGCAGGCA | |
Probe | TTCAGTTGAGCTTGTATG | |
Reverse | CTCAACTGGTGTCGTGGAGTCGGCA | |
EBV BHRF1-2* | Stem-loop RT | CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGGCTATCTG |
Forward | ACACTCCAGCTGGGAAATTCTGTTGCAGCA | |
Probe | TTCAGTTGAGGCTATCTG | |
Reverse | CTCAACTGGTGTCGTGGAGTCGGCA | |
hsa-miR21-5p | TaqMan MicroRNA Assay ID 000397 | |
hsa-miR451 | TaqMan MicroRNA Assay ID 001105 | |
mRNA | ||
IFN-β | Forward | CCTGAAGGCCAAGGAGTACA |
Reverse | AAGCAATTGTCCAGTCCCAG | |
IFITM1 | Forward | ATGTCGTCTGGTCCCTGTTC |
Reverse | GTCATCAGGATGCCCAGAAT | |
IFITM3 | Forward | ATGTCGTCTGGTCCCTGTTC |
Reverse | GGGATGACGATGAGCAGAAT | |
OAS2 | Forward | GGTGAACACCATCTGTGACG |
Reverse | ACCATCGGAGTTGCCTCTTA | |
MX1 | Forward | GATTTTGGGGCTTTCCAGTC |
Reverse | GATGATCAAAGGGATGTGGC | |
TLR3 | Forward | TCACTTGCTCATTCTCCCTT |
Reverse | GACCTCTCCATTCCTGGC | |
TLR7 | Forward | TGCTCTGCTCTCTTCAACCA |
Reverse | AACCATCTAGCCCCAAGGAG | |
TLR9 | Forward | ACACAGCTGCGCAAGCTTAAC |
Reverse | TCGAGTGAGCGGAAGAAGATG | |
PKR | Forward | GGGACCTTGGAACAATGGAT |
Reverse | CCTTGTTCGCTTTCCATCAT | |
RIG-I | Forward | ATATCCGGAAGACCCTGGAC |
Reverse | GAGAAAAAGTGTGGCAGCCT | |
IFIT1 | Forward | TACCTGGACAAGGTGGAGAA |
Reverse | GTGAGGACATGTTGGCTAGA | |
IFIT3 | Forward | CTTACGGCAAGCTGAAGAGT |
Reverse | AATTGCCAGTCCAGAGGAGA | |
IFIT5 | Forward | GGCCATTCTGTTGGAGTTAG |
Reverse | GCTTCTTCAAGCTGGTCCAT | |
MVP | Forward | ATCTGAGGCGTTTGTTGCAG |
Reverse | GGATGCGGATGATGAACTCT | |
La/SSB | Forward | TAATGAAAAGATGGCTGCCC |
Reverse | AAGGTACCCAGCCTTCATCC | |
TIM1 | Forward | CCAGTAGCCACTTCACCATCTTCAC |
Reverse | CGGTGTCATTCCCATCTGTTGTG | |
TIM4 | Forward | ACAGGACAGATGGATGGAATACCC |
Reverse | AGCCTTGTGTGTTTCTGCG | |
GAPDH | Forward | GAGTCAACGGATTTGGTCGTAT |
Reverse | ATGGGTGGAATCATATTGGAAC | |
EBV DNA | ||
EBNA1 QK | Forward | GCCGGTGTGTTCGTATATGG |
Reverse | CAAAACCTCAGCAAATATATGAG | |
Probe 1 | TCTCCCCTTTGGAATGGCCCTG-fluorescein | |
Probe 2 | LCRed640-ACCGGCCCACAACCTG | |
Cloning | ||
EBER1 | Forward | TGCAAGATCTTAATACGACTCACTATAGGAGGACCTACGCTGCCATAGA |
Short Forward* | TGCAAGATCTTAATACGACTCACTATAGGAGGACC | |
Reverse | ACGTTCTAGAAAACATGCGGACCACCAGC |
The T7 promoter sequence is underlined.
Added after 20 rounds of amplification to increase PCR efficiency and yield.