Fig. S4.

Representative dot plots of cells sorted for TCR clonotyping. For enumeration and sorting of D^bPB1-F2₆₂–specific CD8 T cells in 3-, 12-, and 18-mo mice, cells were gated on singlets, live, lymphocytes, dump, and CD3⁺ events. From this gate, CD4⁺ events (Top) were used to define background at a median of 10 events, then CD8⁺ events (Middle) were assessed for D^bPB1-F2₆₂–specific events. Numbers in the dot plots indicate the number of events sorted. Sorted cells were defined as CD44lo vs. CD44hi at the time of sorting by using D^bPB1-F2₆₂–specific tetramer⁺ cells from IAV-infected mice (Bottom). Numbers in the histograms indicate proportion of D^bPB1-F2₆₂–specific cells that are CD44lo or CD44hi.