Fig. 1.

ACR4 interacts with PP2A-3. (A) Schematic representation of ACR4 with key domains and regions used for protein–protein interaction studies. (B) In vitro GST pull-down experiment using GST:PP2A-3 and MBP:ACR4^ICD according to indicated combinations (+). PP2A-3 and ACR4^ICD were detected by Western blotting with anti-GST and anti-MBP antibodies, respectively. (C) In planta YFP pull-down experiment using ACR4:YFP:HA and FLAG:PP2A-3 transiently coexpressed in tobacco leaves by Agrobacterium infiltration according to indicated combinations (+). PP2A-3 and ACR4 were detected by Western blotting with anti-FLAG and anti-HA antibodies, respectively.