Fig. S3.

T-cell–intrinsic Trim24 is essential for i.n. HDM-mediated airway allergy. Chimeric mice with WT or Trim24^−/− T cells were sensitized (day 0) and challenged i.n. (days 7–11) with HDM. *P ≤ 0.05. One of two representative experiments is shown. (A and B) BAL cells were recovered, counted (A), and used for cytospins for differential analysis (B). (C and D) Gene expression in pulmonary tissue of mice with HDM-induced airway allergy. Expression is shown relative to PBS-treated mice. (E) Local lymph node cells were restimulated with HDM. Secreted cytokines were measured in supernatants after 4 d. (F) Circulating IgE measured in the serum of mice with HDM-induced airway allergy. (G) H&E-stained (Left) and AB-PAS–stained (Right) lung sections from chimeric mice with HDM-induced airway allergy.