Fig. S5.

IL1R-expressing T cells are required for IL-1β–exacerbated airway allergy. (A) Chimeric mice were generated by reconstituting irradiated Rag2^−/− mice with 2–5 × 10⁶ bone marrow cells (20% bone marrow from Il1r^−/− or WT donor mice and 80% bone marrow from C57BL/6.Tcra^−/− mice) for 6–8 wk. Chimeric mice with WT or Il1r ^−/− T cells were sensitized with HDM/alum and were challenged with HDM supplemented with IL-1β or not supplemented as indicated. One of two representative experiments is shown. (B) BAL cells were recovered, counted, and used for cytospins for differential analysis. (C) H&E- and AB-PAS–stained lung sections from chimeric mice with HDM-induced airway allergy. (D) Gene expression in pulmonary tissue of mice with HDM-induced airway allergy. Expression is shown relative to Hprt. *P ≤ 0.05.