Fig. 4.

Static conformation of VWF probed by AFM imaging. (A) Images of individual VWF dimers. Conformations of dimers range from fully flexible (normalized stem length of 0) to fully closed (normalized stem length above 1). Numbers in images are values of the normalized stem length. White arrows mark the CK domains, and green arrows mark positions corresponding to potential strong intermonomer interactions. (Scale bar, 30 $\text{nm}$; range of color scale, 2.4 $\text{nm}$.) (B) Distribution of the normalized stem length of wildtype (WT) dimers, showing a peak decaying from zero stem length and a peak centered slightly above 1. (C) Distribution of the normalized stem length of dimers lacking the D4 domain, showing only the peak decaying from zero stem length. (D) Image of a VWF concatamer consisting of four dimeric subunits. Arrows and scales are as in A.